General Information:

Id: 2,812
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
BTO:0002734 16-HBE14o cell, infected with P. aeruginosa strain PA3
article
Reference: Cuzick A et al.(2006) The type III pseudomonal exotoxin U activates the c-Jun NH2-terminal kinase pathway and increases human epithelial interleukin-8 production. Infect. Immun. 74: 4104-4113 [PMID: 16790784]

Interaction Information:

Comment ExoU is the factor responsible for the production of IL-8 in respiratory epithelial cells following infection with P. aeruginosa. The increase in IL-8 produced by ExoU is dependent on the catalytic lipase activity of the protein.
Formal Description
Interaction-ID: 25908

gene/protein

exoU

increases_quantity of

gene/protein

CXCL8

in respiratory epithelial cells
Comment ExoU is responsible for the activation of the SAPK/JNK MAPK pathway in these respiratory epithelial cells.
Formal Description
Interaction-ID: 25909

gene/protein

exoU

increases_activity of

process

MAPK cascade

in respiratory epithelial cells
Comment There was a wild-type PA3 induced marked phosphorylation of SEK1/MKK4, SAPK/JNK, and c-Jun between 4.5 and 7.5 h after infection. Following infection with the delta-exoU mutant of PA3, the phosphorylation of both SEK1/MKK4 and SAPK/JNK was markedly reduced compared to that observed following infection with the wild-type strain. In addition, the phosphorylation of c-Jun was also significantly diminished compared to that observed with the wild-type strain.
Formal Description
Interaction-ID: 25910

gene/protein

exoU

increases_activity of

gene/protein

MAP2K4

in respiratory epithelial cells
Comment There was a wild-type PA3 induced marked phosphorylation of SEK1/MKK4, SAPK/JNK, and c-Jun between 4.5 and 7.5 h after infection. Following infection with the delta-exoU mutant of PA3, the phosphorylation of both SEK1/MKK4 and SAPK/JNK was markedly reduced compared to that observed following infection with the wild-type strain. In addition, the phosphorylation of c-Jun was also significantly diminished compared to that observed with the wild-type strain.
Formal Description
Interaction-ID: 25911

gene/protein

exoU

increases_phosphorylation of

gene/protein

MAP2K4

in respiratory epithelial cells
Comment There was a wild-type PA3 induced marked phosphorylation of SEK1/MKK4, SAPK/JNK, and c-Jun between 4.5 and 7.5 h after infection. Following infection with the delta-exoU mutant of PA3, the phosphorylation of both SEK1/MKK4 and SAPK/JNK was markedly reduced compared to that observed following infection with the wild-type strain. In addition, the phosphorylation of c-Jun was also significantly diminished compared to that observed with the wild-type strain.
Formal Description
Interaction-ID: 25912

gene/protein

exoU

increases_activity of

gene/protein

MAPK9

in respiratory epithelial cells
Drugbank entries Show/Hide entries for MAPK9
Comment There was a wild-type PA3 induced marked phosphorylation of SEK1/MKK4, SAPK/JNK, and c-Jun between 4.5 and 7.5 h after infection. Following infection with the delta-exoU mutant of PA3, the phosphorylation of both SEK1/MKK4 and SAPK/JNK was markedly reduced compared to that observed following infection with the wild-type strain. In addition, the phosphorylation of c-Jun was also significantly diminished compared to that observed with the wild-type strain.
Formal Description
Interaction-ID: 25913

gene/protein

exoU

increases_phosphorylation of

gene/protein

MAPK9

in respiratory epithelial cells
Drugbank entries Show/Hide entries for MAPK9
Comment There was a wild-type PA3 induced marked phosphorylation of SEK1/MKK4, SAPK/JNK, and c-Jun between 4.5 and 7.5 h after infection. Following infection with the delta-exoU mutant of PA3, the phosphorylation of both SEK1/MKK4 and SAPK/JNK was markedly reduced compared to that observed following infection with the wild-type strain. In addition, the phosphorylation of c-Jun was also significantly diminished compared to that observed with the wild-type strain.
Formal Description
Interaction-ID: 25914

gene/protein

exoU

increases_activity of

gene/protein

JUN

in respiratory epithelial cells
Drugbank entries Show/Hide entries for JUN
Comment There was a wild-type PA3 induced marked phosphorylation of SEK1/MKK4, SAPK/JNK, and c-Jun between 4.5 and 7.5 h after infection. Following infection with the delta-exoU mutant of PA3, the phosphorylation of both SEK1/MKK4 and SAPK/JNK was markedly reduced compared to that observed following infection with the wild-type strain. In addition, the phosphorylation of c-Jun was also significantly diminished compared to that observed with the wild-type strain.
Formal Description
Interaction-ID: 25915

gene/protein

exoU

increases_phosphorylation of

gene/protein

JUN

in respiratory epithelial cells
Drugbank entries Show/Hide entries for JUN
Comment It was shown that ExoU produces activation of the c-Jun NH2-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) pathway and that this is required for efficient IL-8 production following infection.
Formal Description
Interaction-ID: 25918

process

MAPK cascade

increases_quantity of

gene/protein

CXCL8

in respiratory epithelial cells