General Information:

Id: 2,655
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
article
Reference: Anderson DM et al.(2011) Ubiquitin and ubiquitin-modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU Mol. Microbiol. 82: 1454-1467 [PMID: 22040088]

Interaction Information:

Comment The results indicate that ExoU is a unique toxin in that it specifically associates with ubiquitin and/or ubiquitylated proteins to activate the enzyme.
Formal Description
Interaction-ID: 25049

gene/protein

UBB

increases_activity of

gene/protein

exoU

Drugbank entries Show/Hide entries for UBB
Comment Recombinant histidine-tagged ExoU was able to bind both monoubiquitin and K48-linked diubiquitin with similar affinities. The affinity of rExoU for K48-linked polyubiquitin is approximately 3.5 fold greater than monoubiquitin or diubiquitin.
Formal Description
Interaction-ID: 25060

gene/protein

UBB

interacts (colocalizes) with

gene/protein

exoU

affinity for polyubiquitin is greater than monoubiquitin or diubiquitin
Drugbank entries Show/Hide entries for UBB
Comment Ubiquitin-like proteins including ISG15, FAT10, SUMO-1 and NEDD8 do not activate rExoU (recombinant ExoU) at the highest concentrations tested.
Formal Description
Interaction-ID: 25062

gene/protein

ISG15

NOT increases_activity of

gene/protein

exoU

Comment Ubiquitin-like proteins including ISG15, FAT10, SUMO-1 and NEDD8 do not activate rExoU (recombinant ExoU) at the highest concentrations tested.
Formal Description
Interaction-ID: 25063

gene/protein

UBD

NOT increases_activity of

gene/protein

exoU

Comment Ubiquitin-like proteins including ISG15, FAT10, SUMO-1 and NEDD8 do not activate rExoU (recombinant ExoU) at the highest concentrations tested.
Formal Description
Interaction-ID: 25064

gene/protein

SUMO1

NOT increases_activity of

gene/protein

exoU

Comment Ubiquitin-like proteins including ISG15, FAT10, SUMO-1 and NEDD8 do not activate rExoU (recombinant ExoU) at the highest concentrations tested.
Formal Description
Interaction-ID: 25065

gene/protein

NEDD8

NOT increases_activity of

gene/protein

exoU

Comment Polyubiquitin molecules associate with and activate ExoU with the greatest efficiency in vitro suggesting that either the size of the cofactor or multiple interaction sites within ExoU are important for phospholipase activity.
Formal Description
Interaction-ID: 25066

gene/protein

exoU

decreases_quantity of

drug/chemical compound

Phospholipid