General Information:

Id: 2,620
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
P. aeruginosa strain PAO1 and PA103
article
Reference: Inclan YF et al.(2011) FimL regulates cAMP synthesis in Pseudomonas aeruginosa. PLoS ONE 6 [PMID: 21264306]

Interaction Information:

Comment FimL regulates cAMP levels. Deletion of fimL correlates with a decrease in cAMP levels in both PAO1 and PA103, strongly supporting a role for FimL in cAMP production in P. aeruginosa.
Formal Description
Interaction-ID: 24866

gene/protein

fimL

affects_quantity of

drug/chemical compound

cAMP

Drugbank entries Show/Hide entries for cAMP
Comment FimL regulation of cAMP could occur by affecting cAMP synthesis, via the adenylate cyclases CyaA or CyaB or by stimulating degradation of cAMP via the phosphodiesterase CpdA. FimL promotes CyaB-dependent cAMP synthesis.
Formal Description
Interaction-ID: 24880

gene/protein

fimL

affects_activity of

gene/protein

cyaB

Comment FimL regulation of cAMP could occur by affecting cAMP synthesis, via the adenylate cyclases CyaA or CyaB or by stimulating degradation of cAMP via the phosphodiesterase CpdA. FimL promotes CyaB-dependent cAMP synthesis.
Formal Description
Interaction-ID: 24883

gene/protein

cyaB

increases_quantity of

drug/chemical compound

cAMP

Drugbank entries Show/Hide entries for cAMP
Comment FimL regulation of cAMP could occur by affecting cAMP synthesis, via the adenylate cyclases CyaA or CyaB or by stimulating degradation of cAMP via the phosphodiesterase CpdA. FimL does not act through CyaA.
Formal Description
Interaction-ID: 24894

gene/protein

fimL

NOT affects_activity of

gene/protein

cyaA

Comment FimL does not affect cyaB transcription or protein levels. It was concluded that FimL modulates CyaB function at a post-translational step.
Formal Description
Interaction-ID: 24895

gene/protein

fimL

NOT affects_expression of

gene/protein

cyaB

Comment FimL does not affect cyaB transcription or protein levels. It was concluded that FimL modulates CyaB function at a post-translational step.
Formal Description
Interaction-ID: 24896

gene/protein

fimL

NOT affects_quantity of

gene/protein

cyaB

Comment Expression of T3SS genes correlates with intracellular cAMP levels. The role of FimL and cAMP on the transcription of a representative T3SS gene, the secreted toxin ExoT was analysed using the panels of isogenic mutants. The corresponding delta-fimL and the delta-cyaB mutants showed greatly reduced P-exoT-lacZ expression, similar to the levels seen in the delta-vfr and delta-cyaA-delta-cyaB mutants.
Formal Description
Interaction-ID: 24901

gene/protein

fimL

affects_expression of

gene/protein

exoT

Comment Expression of T3SS genes correlates with intracellular cAMP levels. The role of FimL and cAMP on the transcription of a representative T3SS gene, the secreted toxin ExoT was analysed using the panels of isogenic mutants. The corresponding delta-fimL and the delta-cyaB mutants showed greatly reduced P-exoT-lacZ expression, similar to the levels seen in the delta-vfr and delta-cyaA-delta-cyaB mutants.
Formal Description
Interaction-ID: 24902

gene/protein

cyaB

affects_expression of

gene/protein

exoT

Comment Expression of T3SS genes correlates with intracellular cAMP levels. The role of FimL and cAMP on the transcription of a representative T3SS gene, the secreted toxin ExoT was analysed using the panels of isogenic mutants. The corresponding delta-fimL and the delta-cyaB mutants showed greatly reduced P-exoT-lacZ expression, similar to the levels seen in the delta-vfr and delta-cyaA-delta-cyaB mutants.
Formal Description
Interaction-ID: 24903

gene/protein

vfr

affects_expression of

gene/protein

exoT

Comment TM (twitching motility) correlates with cAMP levels. All mutants that exhibited decreased cAMP levels and decreased P-exoT-lacZ expression also showed reduced TM. TM was restored to the wild type diameter upon complementation of the corresponding delta-fimL mutant.
Formal Description
Interaction-ID: 24904

gene/protein

fimL

affects_activity of

Comment FimL regulation of cAMP could occur by affecting cAMP synthesis, via the adenylate cyclases CyaA or CyaB or by stimulating degradation of cAMP via the phosphodiesterase CpdA. FimL promotes CyaB-dependent cAMP synthesis.
Formal Description
Interaction-ID: 29405

gene/protein

cpdA

decreases_quantity of

drug/chemical compound

cAMP

Drugbank entries Show/Hide entries for cAMP