General Information:

Id: 2,519
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
TLR5-transfected HEK cells or human neutrophils , incubated with with recombinant AprA of P. aeruginosa strain PAO1
article
Reference: Bardoel BW et al.(2011) Pseudomonas evades immune recognition of flagellin in both mammals and plants. PLoS Pathog. 7 [PMID: 21901099]

Interaction Information:

Comment The authors identified alkaline protease (AprA) of Pseudomonas aeruginosa as a TLR5 signaling inhibitor as evidenced by a marked reduction in IL-8 production and NF-kappaB activation. AprA effectively degrades the TLR5 ligand monomeric flagellin, while polymeric flagellin (involved in bacterial motility) and TLR5 itself resist degradation.
Formal Description
Interaction-ID: 23447

gene/protein

fliC

increases_activity of

gene/protein

TLR5

Comment The authors identified alkaline protease (AprA) of Pseudomonas aeruginosa as a TLR5 signaling inhibitor as evidenced by a marked reduction in IL-8 production and NF-kappaB activation. AprA effectively degrades the TLR5 ligand monomeric flagellin, while polymeric flagellin (involved in bacterial motility) and TLR5 itself resist degradation.
Formal Description
Interaction-ID: 23448

gene/protein

aprA

decreases_quantity of

gene/protein

fliC

Comment The authors identified alkaline protease (AprA) of Pseudomonas aeruginosa as a TLR5 signaling inhibitor as evidenced by a marked reduction in IL-8 production and NF-kappaB activation. AprA effectively degrades the TLR5 ligand monomeric flagellin, while polymeric flagellin (involved in bacterial motility) and TLR5 itself resist degradation.
Formal Description
Interaction-ID: 23449

gene/protein

aprA

decreases_activity of

gene/protein

TLR5

Comment The authors identified alkaline protease (AprA) of Pseudomonas aeruginosa as a TLR5 signaling inhibitor as evidenced by a marked reduction in IL-8 production and NF-kappaB activation. AprA effectively degrades the TLR5 ligand monomeric flagellin, while polymeric flagellin (involved in bacterial motility) and TLR5 itself resist degradation.
Formal Description
Interaction-ID: 23450

gene/protein

aprA

NOT decreases_quantity of

gene/protein

TLR5

Comment The natural occurring alkaline protease inhibitor AprI of P. aeruginosa blocked flagellin degradation by AprA.
Formal Description
Interaction-ID: 23451

gene/protein

aprI

decreases_activity of

gene/protein

aprA

Comment Stimulation of HEK/TLR5 with flagellin triggers the activation of NF-kappaB and the secretion of Interleukin 8 (IL-8). AprA prevents flagellin-induced IL-8 production by HEK/TLR5 cells.
Formal Description
Interaction-ID: 23452

gene/protein

fliC

increases_quantity of

gene/protein

CXCL8

Comment Stimulation of HEK/TLR5 with flagellin triggers the activation of NF-kappaB and the secretion of Interleukin 8 (IL-8). AprA prevents flagellin-induced IL-8 production by HEK/TLR5 cells.
Formal Description
Interaction-ID: 23453

gene/protein

aprA

decreases_quantity of

gene/protein

CXCL8