General Information:

Id: 2,336 (click here to show other Interactions for entry)
Diseases: Pseudomonas aeruginosa diseases
pathogen-host system
Pseudomonas aeruginosa/mammalia
review
Reference: Hauser AR(2009) The type III secretion system of Pseudomonas aeruginosa: infection by injection. Nat. Rev. Microbiol. 7: 654-665 [PMID: 19680249]

Interaction Information:

Comment The membrane localization domain (MLD) (residues 51-72) of the P. aeruginosa effector ExoS is responsible for the initial transient localization to the plasma membrane of the host cell. Subsequent trafficking to the Golgi and endoplasmic reticulum in the perinuclear region of the cell occurs in association with markers for Rab5-containing early endosomes and Rab6- and Rab9-containing late endosomes.
Formal Description
Interaction-ID: 20362

gene/protein

exoS

interacts (colocalizes) with

gene/protein

RAB5

Comment The membrane localization domain (MLD) (residues 51-72) of the P. aeruginosa effector ExoS is responsible for the initial transient localization to the plasma membrane of the host cell. Subsequent trafficking to the Golgi and endoplasmic reticulum in the perinuclear region of the cell occurs in association with markers for Rab5-containing early endosomes and Rab6- and Rab9-containing late endosomes.
Formal Description
Interaction-ID: 20363

gene/protein

exoS

interacts (colocalizes) with

gene/protein

RAB6

Drugbank entries Show/Hide entries for RAB6
Comment The membrane localization domain (MLD) (residues 51-72) of the P. aeruginosa effector ExoS is responsible for the initial transient localization to the plasma membrane of the host cell. Subsequent trafficking to the Golgi and endoplasmic reticulum in the perinuclear region of the cell occurs in association with markers for Rab5-containing early endosomes and Rab6- and Rab9-containing late endosomes.
Formal Description
Interaction-ID: 20364

gene/protein

exoS

interacts (colocalizes) with

gene/protein

RAB9

Comment ExoS is a GTPase activating protein. Residues 96-233 of ExoS form a GAP domain that targets RhoA, Rac1, and Cdc42, small GTPases that maintain the organization of the actin cytoskeleton. These regulatory GTPases normally switch between an active GTP-bound form and an inactive GDP-bound form, but the ExoS GAP domain biases the switch towards the ADP-bound inactive form, leading to disruption of the actin cytoskeleton.
Formal Description
Interaction-ID: 20367

gene/protein

exoS

decreases_activity of

gene/protein

RHOA

Drugbank entries Show/Hide entries for RHOA
Comment ExoS is a GTPase activating protein. Residues 96-233 of ExoS form a GAP domain that targets RhoA, Rac1, and Cdc42, small GTPases that maintain the organization of the actin cytoskeleton. These regulatory GTPases normally switch between an active GTP-bound form and an inactive GDP-bound form, but the ExoS GAP domain biases the switch towards the ADP-bound inactive form, leading to disruption of the actin cytoskeleton.
Formal Description
Interaction-ID: 20368

gene/protein

exoS

decreases_activity of

gene/protein

RAC1

Drugbank entries Show/Hide entries for RAC1
Comment ExoS is a GTPase activating protein. Residues 96-233 of ExoS form a GAP domain that targets RhoA, Rac1, and Cdc42, small GTPases that maintain the organization of the actin cytoskeleton. These regulatory GTPases normally switch between an active GTP-bound form and an inactive GDP-bound form, but the ExoS GAP domain biases the switch towards the ADP-bound inactive form, leading to disruption of the actin cytoskeleton.
Formal Description
Interaction-ID: 20369

gene/protein

exoS

decreases_activity of

gene/protein

CDC42

Drugbank entries Show/Hide entries for CDC42
Comment ExoS is a GTPase activating protein. Residues 96-233 of ExoS form a GAP domain that targets RhoA, Rac1, and Cdc42, small GTPases that maintain the organization of the actin cytoskeleton. These regulatory GTPases normally switch between an active GTP-bound form and an inactive GDP-bound form, but the ExoS GAP domain biases the switch towards the ADP-bound inactive form, leading to disruption of the actin cytoskeleton.
Formal Description
Interaction-ID: 20370

gene/protein

exoS

decreases_activity of

Comment ExoS catalyzes the transfer of ADP-ribose to many different host proteins. Substrates of special interest are Ras and the ezrin, radixin and moesin (ERM) family of proteins.
Formal Description
Interaction-ID: 20373

gene/protein

exoS

affects_activity of

gene/protein

HRAS

Drugbank entries Show/Hide entries for HRAS
Comment ExoS catalyzes the transfer of ADP-ribose to many different host proteins. Substrates of special interest are Ras and the ezrin, radixin and moesin (ERM) family of proteins. ADP-ribosylation of ERM proteins prevents their phosphorylation, resulting in inactivation.
Formal Description
Interaction-ID: 20374

gene/protein

exoS

decreases_activity of

gene/protein

EZR

Comment ExoS catalyzes the transfer of ADP-ribose to many different host proteins. Substrates of special interest are Ras and the ezrin, radixin and moesin (ERM) family of proteins. ADP-ribosylation of ERM proteins prevents their phosphorylation, resulting in inactivation.
Formal Description
Interaction-ID: 20375

gene/protein

exoS

decreases_activity of

gene/protein

RDX

Drugbank entries Show/Hide entries for RDX
Comment ExoS catalyzes the transfer of ADP-ribose to many different host proteins. Substrates of special interest are Ras and the ezrin, radixin and moesin (ERM) family of proteins. ADP-ribosylation of ERM proteins prevents their phosphorylation, resulting in inactivation.
Formal Description
Interaction-ID: 20376

gene/protein

exoS

decreases_activity of

gene/protein

MSN

Comment ExoS is a GTPase activating protein. Residues 96-233 of ExoS form a GAP domain that targets RhoA, Rac1, and Cdc42, small GTPases that maintain the organization of the actin cytoskeleton. These regulatory GTPases normally switch between an active GTP-bound form and an inactive GDP-bound form, but the ExoS GAP domain biases the switch towards the ADP-bound inactive form, leading to disruption of the actin cytoskeleton.
Formal Description
Interaction-ID: 23446

gene/protein

exoS

increases_activity of

Comment The membrane localization domain (MLD) (residues 51-72) of the P. aeruginosa effector ExoS is responsible for the initial transient localization to the plasma membrane of the host cell. Subsequent trafficking to the Golgi and endoplasmic reticulum in the perinuclear region of the cell occurs in association with markers for Rab5-containing early endosomes and Rab6- and Rab9-containing late endosomes.
Formal Description
Interaction-ID: 38846

gene/protein

exoS

is localized in

cellular component

host cell plasma membrane

transient localization