General Information:

Id: 3,873 (click here to show other Interactions for entry)
Mus musculus
B6.SJL-Ptprc a/BoAiTac
Reference: Nemeth MJ et al.(2007) Wnt5a inhibits canonical Wnt signaling in hematopoietic stem cells and enhances repopulation Proc. Natl. Acad. Sci. U.S.A. 104 [PMID: 17881570]

Interaction Information:

Comment Wnt5a inhibits canonical Wnt signaling induced by Wnt3a by 50%, indicating that Wnt3a and Wnt5a have antagonistic effects on the canonical Wnt pathway in HSCs in vitro. [Method: Il-7Ralpha- KSL cells (isolated from TOPGAL mice) cultured with recombinant Wnt3a or Wnt5a]
Formal Description
Interaction-ID: 39001

gene/protein

Wnt5a

decreases_activity of

gene/protein

Wnt3a

in CD127-KSL cells in vitro
Comment Wnt5a inhibits canonical Wnt signaling induced by Wnt3a by 50%, indicating that Wnt3a and Wnt5a have antagonistic effects on the canonical Wnt pathway in HSCs in vitro. [Method: Il-7Ralpha- KSL cells (isolated from TOPGAL mice) cultured with recombinant Wnt3a or Wnt5a]
Formal Description
Interaction-ID: 39010

gene/protein

Wnt3a

increases_activity of

in CD127-KSL cells in vitro
Comment Under serum-free conditions, the presence of Wnt3a significantly reduced total cell expansion 3-fold after 4 days in culture compared with control conditions (SCF and Flt3L alone). However, Wnt5a either alone (12-fold) or in combination with Wnt3a (16-fold) also significantly reduced cell expansion compared with control conditions. [Method: Il-7Ralpha- KSL cells (isolated from TOPGAL mice) cultured with recombinant Wnt3a or Wnt5a]
Formal Description
Interaction-ID: 39028

gene/protein

Wnt3a

decreases_quantity of

Immunophenotype

HSC (CD127-KSL)

in vitro
Comment Both (Wnt3a: 2.4-fold; Wnt5a: 3-fold) significantly increased the percentage of total cells actively undergoing apoptosis from 1% to 3%. [Method: Il-7Ralpha- KSL cells (isolated from TOPGAL mice) cultured with recombinant Wnt3a or Wnt5a, Annexin V and PI staining]
Formal Description
Interaction-ID: 39030

gene/protein

Wnt3a

increases_activity of

in CD127-KSL cells in vitro
Comment Wnt3a does not enhance short-term or long-term repopulation activity of l-7Ralpha- KSL (CD127-) cells. [Method: Il-7Ralpha- KSL cells cultured with recombinant Wnt3a for 6 d, 6 or 16 weeks after transplantation]
Formal Description
Interaction-ID: 39032

gene/protein

Wnt3a

NOT affects_activity of

process

repopulation >16 weeks

of CD127-KSL cells in vivo
Comment Wnt3a does not enhance short-term or long-term repopulation activity of l-7Ralpha- KSL (CD127-) cells. [Method: Il-7Ralpha- KSL cells cultured with recombinant Wnt3a for 6 d, 6 or 16 weeks after transplantation]
Formal Description
Interaction-ID: 39033

gene/protein

Wnt3a

NOT affects_activity of

process

repopulation <12 weeks

of CD127-KSL cells in vivo
Comment Wnt3a induced a significant (2.5-fold) increase in c-myc mRNA compared with control and Wnt5a abrogated this induction. [Method: Il-7Ralpha- KSL cells cultured with recombinant Wnt5a, RT-PCR]
Formal Description
Interaction-ID: 39039

gene/protein

Wnt3a

increases_expression of

gene/protein

Myc

in CD127-KSL cells